Japanese
researchers said on Tuesday they had developed a new method to detect
the presence of the Ebola virus in 30 minutes, with technology that
could allow doctors to quickly diagnose infection.
Professor
Jiro Yasuda and his team at Nagasaki University say their process is
also cheaper than the system currently in use in west Africa where the
virus has already killed more than 1,500 people.
“The new
method is simpler than the current one and can be used in countries
where expensive testing equipment is not available,” Yasuda told AFP by
telephone.
“We have yet to receive any questions or requests, but we are pleased to offer the system, which is ready to go,” he said.
Yasuda
said the team had developed what he called a “primer,” which amplifies
only those genes specific to the Ebola virus found in a blood sample or
other bodily fluid.
Using
existing techniques, ribonucleic acid (RNA) — biological molecules used
in the coding of genes — is extracted from any viruses present in a
blood sample.
This is
then used to synthesise the viral DNA, which can be mixed with the
primers and then heated to 60-65 degrees Celsius (140-149 Fahrenheit).
If Ebola
is present, DNA specific to the virus is amplified in 30 minutes due to
the action of the primers. The by-products from the process cause the
liquid to become cloudy, providing visual confirmation, Yasuda said.
Currently,
a method called polymerase chain reaction, or PCR, is widely used to
detect the Ebola virus, which requires doctors to heat and cool samples
repeatedly and takes up to two hours.
“The new
method only needs a small, battery-powered warmer and the entire system
costs just tens of thousands of yen (hundreds of dollars), which
developing countries should be able to afford,” he added.
The
National Biotechnology Development Agency, Abuja, earlier said it had
developed primers and probes for precise detection of Ebola virus
disease, following the release of the genomic sequence of the Zaire
strain of the virus.
The agency
stated that it collaborated with its genomic partners in South Korea to
develop the primers, in order to facilitate early laboratory diagnosis
and adequate response.
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